Searchable abstracts of presentations at key conferences on calcified tissues

ba0001pp210 | Cell biology: osteoblasts and bone formation | ECTS2013

Open source software for semi-automated histomorphometry of bone resorption and formation parameters

van 't Hof Rob , Rose Lorraine , Daroszewska Anna

Histomorphometric analysis is an essential technique to measure bone formation and resorption parameters. Here we present three novel open source image analysis packages that allow the rapid semi-automated analysis of histomorphometric bone resorption, osteoid, and calcein double labelling parameters.Mice were injected with calcein 5 and 2 days before killing. Tibia and vertebrae were fixed in formalin, embedded in methylmethacrylate and the blocks secti...